pcDNA3-HER2-DMVF-181-N
(Plasmid #87622)

• Purpose: N-terminal Dronpa fragment fused to the C-terminus of full-length HER2; for BiFC.
• Depositing Lab: Jin Zhang
• Publication: Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi: 10.1016/j.celrep.2015.12.036. Epub 2015 Dec 31.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 9768
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HER2-DMVF181-N
• Alt name: ERBB2
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 4395
• Entrez Gene: ERBB2 (a.k.a. CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, NEU, NGL, TKR1, VSCN2, c-ERB-2, c-ERB2, p185(erbB2))
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: BGH reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: HER2 cDNA was provided by Dr. Dan Leahy (Johns Hopkins).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS results found I655V within the HER2 translation compared to NP_004439.2.