pcDNA3-HER3-DMVF-181-R
(Plasmid #87621)

• Purpose: C-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.
• Depositing Lab: Jin Zhang
• Publication: Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi: 10.1016/j.celrep.2015.12.036. Epub 2015 Dec 31.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 9669
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HER3-DMVF181-C
• Alt name: Erbb3
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 4254
• Entrez Gene: ERBB3 (a.k.a. ErbB-3, FERLK, HER3, LCCS2, MDA-BF-1, VSCN1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-HER3-DMVF-181-R pcDNA3-HER2-DMVF181-N
• A portion of this plasmid was derived from a plasmid made by: HER3 cDNA was provided by Dr. Dan Leahy (Johns Hopkins).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS results found I655V within the HER2 translation compared to NP_004439.2