pcDNA3.1-STIM1-Venus-173-C
(Plasmid #87619)

• Purpose: Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.
• Depositing Lab: Jin Zhang
• Publication: Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi: 10.1016/j.celrep.2015.12.036. Epub 2015 Dec 31.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7707
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: STIM1-Venus173-C
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 2295
• Entrez Gene: STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3.1-STIM1-Venus173-C
• A portion of this plasmid was derived from a plasmid made by: STIM1 cDNA provided by Dr. Paul Worley (Johns Hopkins)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry