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Halo-Tev-Akt3 C119S-2X Flag
(Plasmid #87608)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87608 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+8
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 6595
  • Vector type
    Mammalian Expression ; Zebrafish (Danio rerio) expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AKT3 isoform 2
  • Alt name
    Protein Kinase B-gamma
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • Mutation
    cysteine 119 residue mutated to serine
  • GenBank ID
    NM_001206729.1
  • Entrez Gene
    AKT3 (a.k.a. MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2)
  • Tags / Fusion Proteins
    • His-Halo-Tev (N terminal on insert)
    • 2 tandem flag tags (C terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Halo-Tev-Akt3 C119S-2X Flag was a gift from Yimon Aye (Addgene plasmid # 87608 ; http://n2t.net/addgene:87608 ; RRID:Addgene_87608)
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