Halo-Tev-Akt3 WT-2X Flag
(Plasmid #87607)

• Purpose: expresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of human Akt3 protein
• Depositing Lab: Yimon Aye
• Publication: Aye Lab Halo-Tev-Akt3 plasmid (unpublished)

Backbone

• Vector backbone: pCS2+8
• Backbone size w/o insert (bp): 4095
• Total vector size (bp): 6595
• Vector type: Mammalian Expression ; Zebrafish (Danio rerio) expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Akt3 isoform 2
• Alt name: Protein Kinase B-gamma
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• Mutation: Akt3 isoform 2
• GenBank ID: NM_001206729.1
• Entrez Gene: AKT3 (a.k.a. MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2)
• Promoter: SP6
• Tags / Fusion Proteins:
  • His-Halo-Tev (N terminal on insert)
  • 2 tandem flag tags (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: SP6 fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry