pRS413 Prp5 N399D
(Plasmid
#87602)
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PurposeEncodes Prp5 N399D for expression in yeast
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87602 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS413
- Backbone size w/o insert (bp) 4970
- Total vector size (bp) 8524
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePRP5
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2550
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MutationN399D
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GenBank IDNP_009796.1
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Entrez GenePRP5 (a.k.a. YBR237W, RNA5)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer ACCGCGGTGGCGGCCGCTCTAGAATTTAAACCCGGATTCTGCCTCAATG
- 3′ sequencing primer GATATCGAATTCCTGCAGCCCGGGGGATCCCCCTATGAATTGATGTGATAAACTTACC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS413 Prp5 N399D was a gift from Aaron Hoskins (Addgene plasmid # 87602 ; http://n2t.net/addgene:87602 ; RRID:Addgene_87602) -
For your References section:
SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast. Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA. Nucleic Acids Res. 2017 Jan 6. pii: gkw1349. doi: 10.1093/nar/gkw1349. 10.1093/nar/gkw1349 PubMed 28062854