PI(LM)-Rac1(WT)dc1.FLARE
(Plasmid #87590)

• Purpose: PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor
• Depositing Lab: Klaus Hahn
• Publication: Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444.

Backbone

• Vector backbone: pTriEx
• Backbone size w/o insert (bp): 5238
• Total vector size (bp): 7228
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: LOV2
• Species: Synthetic
• Insert Size (bp): 447
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: aagtatcgggccctttgtgc
• 3′ sequencing primer: GGCAGCCTGCACCTGAGGTTAATCAC

Gene/Insert 2

• Gene/Insert name: PBD
• Promoter: CMV
• Tag / Fusion Protein:
  • YPet

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: aagtatcgggccctttgtgc
• 3′ sequencing primer: GGCAGCCTGCACCTGAGGTTAATCAC

Gene/Insert 3

• Gene/Insert name: Rac1
• Promoter: CMV
• Tag / Fusion Protein:
  • dTurq

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: TCGATCTCAGTGGTATTTGTG
• 3′ sequencing primer: AAGTTCTTTTGCCGCCTCATC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Addgene's quality control sequencing finds an additional T to I amino acid residue substitution in the LOV2 domain of this construct. The affect of this residue change is unknown.