3222 pSFFV-neo Bad S136A
(Plasmid
#8759)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8759 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSFFV-neo
- Backbone size w/o insert (bp) 7200
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBad
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SpeciesM. musculus (mouse)
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Insert Size (bp)615
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MutationS136A
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GenBank IDNM_007522
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Entrez GeneBad (a.k.a. AI325008, Bbc2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SFFV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3222 pSFFV-neo Bad S136A was a gift from Stanley Korsmeyer (Addgene plasmid # 8759 ; http://n2t.net/addgene:8759 ; RRID:Addgene_8759) -
For your References section:
p70S6 kinase signals cell survival as well as growth, inactivating the pro-apoptotic molecule BAD. Harada H, Andersen JS, Mann M, Terada N, Korsmeyer SJ. Proc Natl Acad Sci U S A 2001 Aug 14;98(17):9666-70. Epub 2001 Aug 7. 10.1073/pnas.171301998 PubMed 11493700