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Addgene

3205 pCRII Mcl-1 fragment
(Plasmid #8757)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8757 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR II
  • Backbone size w/o insert (bp) 3948
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mcl-1 cDNA PCR fragment
  • Alt name
    Mcl-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    280
  • Mutation
    Base pairs 943-1223.
  • GenBank ID
    NM_008562
  • Entrez Gene
    Mcl1 (a.k.a. Gm52627, Mcl-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use as an external probe for genomic Southerns (for in situ use NotI). Amp and Kan resistance markers.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3205 pCRII Mcl-1 fragment was a gift from Stanley Korsmeyer (Addgene plasmid # 8757 ; http://n2t.net/addgene:8757 ; RRID:Addgene_8757)

  • For your References section:

    Mcl-1 deficiency results in peri-implantation embryonic lethality. Rinkenberger JL, Horning S, Klocke B, Roth K, Korsmeyer SJ. Genes Dev 2000 Jan 1;14(1):23-7. PubMed 10640272
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