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Addgene

3187 pSFFV-neo Bad cDNA ORF
(Plasmid #8756)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8756 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSFFV-neo
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BAD
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    615
  • GenBank ID
    NM_007522
  • Entrez Gene
    Bad (a.k.a. AI325008, Bbc2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SFFV-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gabriel Nunez

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3187 pSFFV-neo Bad cDNA ORF was a gift from Stanley Korsmeyer (Addgene plasmid # 8756 ; http://n2t.net/addgene:8756 ; RRID:Addgene_8756)

  • For your References section:

    Serine phosphorylation of death agonist BAD in response to survival factor results in binding to 14-3-3 not BCL-X(L). Zha J, Harada H, Yang E, Jockel J, Korsmeyer SJ. Cell 1996 Nov 15;87(4):619-28. 10.1016/S0092-8674(00)81382-3 PubMed 8929531
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