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Addgene

His-Keap1-Tev-Halo
(Plasmid #87545)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87545 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIR
  • Backbone size w/o insert (bp) 6470
  • Total vector size (bp) 8370
  • Modifications to backbone
    Contains TRAF6 in the 3'-UTR
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Keap1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2859
  • GenBank ID
    NM_203500.1
  • Entrez Gene
    KEAP1 (a.k.a. INrf2, KLHL19)
  • Tags / Fusion Proteins
    • His-Tag (N terminal on insert)
    • Halo (C terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-Keap1-Tev-Halo was a gift from Yimon Aye (Addgene plasmid # 87545 ; http://n2t.net/addgene:87545 ; RRID:Addgene_87545)

  • For your References section:

    T-REX on-demand redox targeting in live cells. Parvez S, Long MJ, Lin HY, Zhao Y, Haegele JA, Pham VN, Lee DK, Aye Y. Nat Protoc. 2016 Dec;11(12):2328-2356. doi: 10.1038/nprot.2016.114. Epub 2016 Oct 27. 10.1038/nprot.2016.114 PubMed 27809314
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