3185 pBS Bax cDNA
(Plasmid #8754)

• Depositing Lab: Stanley Korsmeyer
• Publication: Knudson et al Science 1995 Oct 6;270(5233):96-9.

Backbone

• Vector backbone: pBluescript II KS(+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bax
• Species: M. musculus (mouse)
• Insert Size (bp): 800
• GenBank ID: NM_007527
• Entrez Gene: Bax

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

for in situ probes: antisense - cut with PstI and use T7 polymerase. sense - cut with SalI and use T3 polymerase.