3158 pSFFV-neo Bad cDNA
(Plasmid #8753)

• Depositing Lab: Stanley Korsmeyer
• Publication: Yang et al Cell 1995 Jan 27;80(2):285-91.

Backbone

• Vector backbone: pSFFV-neo
• Backbone size w/o insert (bp): 7200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BAD
• Species: M. musculus (mouse)
• Insert Size (bp): 615
• GenBank ID: NM_007522
• Entrez Gene: Bad (a.k.a. AI325008, Bbc2)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SFFV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gabriel Nunez

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The expression plasmid pSFFVHA-Bad was constructed by using PCR primers, which incorporated the 9 codons of the HA epitope onto the 5' end of Bad, and it was ligated into pSFFV vector at the EcoRI site.