3155 pGEM-4Z Bcl-2 cDNA G145A
(Plasmid
#8751)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8751 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-4Z
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBcl-2
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SpeciesH. sapiens (human)
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Insert Size (bp)720
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MutationGlycine 145 mutated to Alanine
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GenBank IDNM_000633
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Entrez GeneBCL2 (a.k.a. Bcl-2, PPP1R50)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3155 pGEM-4Z Bcl-2 cDNA G145A was a gift from Stanley Korsmeyer (Addgene plasmid # 8751 ; http://n2t.net/addgene:8751 ; RRID:Addgene_8751) -
For your References section:
BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax. Yin XM, Oltvai ZN, Korsmeyer SJ. Nature 1994 May 26;369(6478):321-3. 10.1038/369321a0 PubMed 8183370