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Addgene

3149 pSFFV-neo Bcl-2 cDNA
(Plasmid #8750)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8750 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSFFV-neo
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bcl-2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    720
  • GenBank ID
    NM_177410
  • Entrez Gene
    Bcl2 (a.k.a. Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SFFV-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gabriel Nunez
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3149 pSFFV-neo Bcl-2 cDNA was a gift from Stanley Korsmeyer (Addgene plasmid # 8750 ; http://n2t.net/addgene:8750 ; RRID:Addgene_8750)

  • For your References section:

    Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Oltvai ZN, Milliman CL, Korsmeyer SJ. Cell 1993 Aug 27;74(4):609-19. 10.1016/0092-8674(93)90509-O PubMed 8358790
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