3120 pSFFV-neo Bcl-XL
(Plasmid #8749)

• Depositing Lab: Stanley Korsmeyer
• Publication: Chao et al J Exp Med 1995 Sep 1;182(3):821-8.

Backbone

• Vector backbone: pSFFV-neo
• Backbone size w/o insert (bp): 7200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bcl-xl
• Species: H. sapiens (human)
• Insert Size (bp): 700
• GenBank ID: NM_138578
• Entrez Gene: BCL2L1 (a.k.a. BCL-XL/S, BCL2L, BCLX, Bcl-X, PPP1R52)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: SFFV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gabriel Nunez
• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry