3063 pBS Hox11 genomic
(Plasmid
#8748)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8748 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript KS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHox11 (genomic fragment)
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Alt nameHox11
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SpeciesM. musculus (mouse)
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Insert Size (bp)550
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GenBank IDS38742
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Entrez GeneTlx1 (a.k.a. Hox-11, Hox11, Tlx-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
From primer A1.5 (just 3' of homeobox) to SalI site.
For use in whole-mount and regular in situ probe generation.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3063 pBS Hox11 genomic was a gift from Stanley Korsmeyer (Addgene plasmid # 8748 ; http://n2t.net/addgene:8748 ; RRID:Addgene_8748) -
For your References section:
Hox11 controls the genesis of the spleen. Roberts CW, Shutter JR, Korsmeyer SJ. Nature 1994 Apr 21;368(6473):747-9. 10.1038/368747a0 PubMed 7908720