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Addgene

2842 pBS Bcl-2 5'genomic
(Plasmid #8747)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8747 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bcl-2 5' genomic
  • Alt name
    Bcl-2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4200
  • GenBank ID
    NM_177410
  • Entrez Gene
    Bcl2 (a.k.a. Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    2842 pBS Bcl-2 5'genomic was a gift from Stanley Korsmeyer (Addgene plasmid # 8747 ; http://n2t.net/addgene:8747 ; RRID:Addgene_8747)
  • For your References section:

    Bcl-2-deficient mice demonstrate fulminant lymphoid apoptosis, polycystic kidneys, and hypopigmented hair. Veis DJ, Sorenson CM, Shutter JR, Korsmeyer SJ. Cell 1993 Oct 22;75(2):229-40. 10.1016/0092-8674(93)80065-M PubMed 8402909