2842 pBS Bcl-2 5'genomic
(Plasmid
#8747)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 8747 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript KS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBcl-2 5' genomic
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Alt nameBcl-2
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SpeciesM. musculus (mouse)
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Insert Size (bp)4200
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GenBank IDNM_177410
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Entrez GeneBcl2 (a.k.a. Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
2842 pBS Bcl-2 5'genomic was a gift from Stanley Korsmeyer (Addgene plasmid # 8747 ; http://n2t.net/addgene:8747 ; RRID:Addgene_8747) -
For your References section:
Bcl-2-deficient mice demonstrate fulminant lymphoid apoptosis, polycystic kidneys, and hypopigmented hair. Veis DJ, Sorenson CM, Shutter JR, Korsmeyer SJ. Cell 1993 Oct 22;75(2):229-40. 10.1016/0092-8674(93)80065-M PubMed 8402909
Map uploaded by the depositor.