2674 pBS Bcl-2 P1+P2
(Plasmid #8746)

• Depositing Lab: Stanley Korsmeyer
• Publication: Young et al Mol Cell Biol 1993 Jun;13(6):3686-97.

Backbone

• Vector backbone: pBluescript II KS(-)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bcl-2 P1+P2
• Alt name: Bcl-2
• Species: H. sapiens (human)
• Insert Size (bp): 5300
• GenBank ID: NM_000633
• Entrez Gene: BCL2 (a.k.a. Bcl-2, PPP1R50)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

bcl-2 is from a genomic clone isolated from a normal human fibroblast library. To construct P1+P2Neo, a 279-bp polymerase chain reaction (PCR) product including the bcl-2 5'-untranslated region sequence from just 5' to the AccI site through base -1, relative to the initiation codon, (designated AccI/-1), was cloned into pBluescript KS(-) (Stratagene), using primergenerated 5' XhoI and 3' HindIII sites. Next, a 486-bp XhoI-AccI fragment from the 5'-untranslated region was cloned into this plasmid, producing a 756-bp XhoI/-1 (HindIII) clone. Addition of an approximately 2.2-kb XhoI fragment to this construct created a 3.0-kb bcl-2 P1+P2 clone. Finally, a 2.3-kb HindIII-BamHI fragment from RSVNeo (20) including the neomycin phosphotransferase (neo) gene and SV40 early splice and polyadenylation signals was inserted into the P1+P2 clone to produce P1+P2Neo.