alpha1Ddelta-G12-FRB
(Plasmid
#87454)
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Purposerapamycin-mediated heterodimerization between IQV and PCRD/DCRD from C-terminal of LTCCs
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87454 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA6
- Backbone size w/o insert (bp) 5069
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namea1D short variants
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SpeciesR. norvegicus (rat)
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Insert Size (bp)4875
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Mutationdelete KYPAKNTTIALQMLERML
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Entrez GeneCacna1d
- Promoter CMV
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Tag
/ Fusion Protein
- FRB (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe I (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
FRB (279 bp)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
alpha1Ddelta-G12-FRB was a gift from Xiaodong Liu (Addgene plasmid # 87454 ; http://n2t.net/addgene:87454 ; RRID:Addgene_87454) -
For your References section:
Cooperative and acute inhibitionby multiple C-terminal motifs of L-type Ca2+ channels. Liu N, Yang Y, Ge L, Liu M, Colecraft HM, Liu X. Elife. 2017 Jan 6;6. pii: e21989. doi: 10.7554/eLife.21989. 10.7554/eLife.21989 PubMed 28059704