FRB-CFP-ras
(Plasmid
#87451)
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Purposerapamycin binding motif of mTOR (FRB) anchored to the plasma membrane
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87451 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5388
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFRB
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SpeciesH. sapiens (human)
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Insert Size (bp)282
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MutationFKBP and rapamycin binding domain only
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Entrez GeneMTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
- Promoter CMV
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Tags
/ Fusion Proteins
- ras fragment (C terminal on insert)
- CFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Xho I (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ras fragment (60 bp)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FRB-CFP-ras was a gift from Xiaodong Liu (Addgene plasmid # 87451 ; http://n2t.net/addgene:87451 ; RRID:Addgene_87451) -
For your References section:
Cooperative and acute inhibitionby multiple C-terminal motifs of L-type Ca2+ channels. Liu N, Yang Y, Ge L, Liu M, Colecraft HM, Liu X. Elife. 2017 Jan 6;6. pii: e21989. doi: 10.7554/eLife.21989. 10.7554/eLife.21989 PubMed 28059704
Map uploaded by the depositor.
