CFP-PCRD-FRB
(Plasmid
#87449)
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Purposerapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87449 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA4
- Backbone size w/o insert (bp) 5209
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePCRD (proximal C-terminal regulatory domain)
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SpeciesH. sapiens (human)
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Insert Size (bp)384
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Entrez GeneCACNA1F (a.k.a. AIED, COD3, COD4, CORDX, CORDX3, CSNB2, CSNB2A, CSNBX2, Cav1.4, Cav1.4alpha1, JM8, JMC8, OA2)
- Promoter CMV
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Tags
/ Fusion Proteins
- FRB (C terminal on insert)
- 6xHis-T7 (N terminal on insert)
- CFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn I (not destroyed)
- 3′ cloning site Xho I (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
FRB (279 bp)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CFP-PCRD-FRB was a gift from Xiaodong Liu (Addgene plasmid # 87449 ; http://n2t.net/addgene:87449 ; RRID:Addgene_87449) -
For your References section:
Cooperative and acute inhibitionby multiple C-terminal motifs of L-type Ca2+ channels. Liu N, Yang Y, Ge L, Liu M, Colecraft HM, Liu X. Elife. 2017 Jan 6;6. pii: e21989. doi: 10.7554/eLife.21989. 10.7554/eLife.21989 PubMed 28059704