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pCSDest2-NmeCas9-NLS-3XHA-NLS
(Plasmid #87448)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87448 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSDest2
  • Backbone size w/o insert (bp) 4358
  • Total vector size (bp) 7604
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nme Cas9
  • Species
    Neisseria meningitidis
  • Insert Size (bp)
    3246
  • Promoter CMV
  • Tags / Fusion Proteins
    • SV40 NLS (C terminal on insert)
    • 3XHA (C terminal on insert)
    • cMyc-like NLS (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATTTAGGTGACACTATAGA
  • 3′ sequencing primer CAGGAAACAGCTATGACCATG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSDest2-NmeCas9-NLS-3XHA-NLS was a gift from Erik Sontheimer (Addgene plasmid # 87448 ; http://n2t.net/addgene:87448 ; RRID:Addgene_87448)

  • For your References section:

    Naturally Occurring Off-Switches for CRISPR-Cas9. Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. 10.1016/j.cell.2016.11.017 PubMed 27984730
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