pCMV-HF-BE3
(Plasmid #87439)

• Purpose: Expresses HF-BE3 with C-terminal NLS in mammalian cells
• Depositing Lab: David Liu
• Publication: Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790.

Backbone

• Vector backbone: pCMV
• Backbone size w/o insert (bp): 2999
• Total vector size (bp): 8532
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HF-BE3
• Alt name: rAPOBEC1-XTEN-nHF-Cas9-GGS-UGI-GGS-NLS
• Species: H. sapiens (human), R. norvegicus (rat); Bacillus phage PBS2
• Insert Size (bp): 5133
• Mutation: Mammalian codon-optimized Cas9-HF
• Entrez Gene: Apobec1 (a.k.a. REPR, apobec-1)
• Promoter: CMV

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TAATACGACTCACTATAGG
• 3′ sequencing primer: CCTTGCTGTCCTGCCCCACCCCACC

Resource Information

• Supplemental Documents:
  • ACK145_pCMV_rAPOBEC1_XTEN_HFCas9n_UGI_NLS Copy.gb
• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry