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Addgene

pKT0146
(Plasmid #8742)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8742 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a-link
  • Backbone size w/o insert (bp) 5416
  • Vector type
    Yeast Expression
  • Selectable markers
    S. pombe HIS5

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tdimer2
  • Alt name
    pKT146 (pFA6a–link–tdimer2–SpHIS5)
  • Tag / Fusion Protein
    • Codon optimized linker (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full name of this plasmid is pFA6a-link-tdimer2-SpHIS5. tdimer2 is described in Campbell et al., 2002.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKT0146 was a gift from Kurt Thorn (Addgene plasmid # 8742 ; http://n2t.net/addgene:8742 ; RRID:Addgene_8742)

  • For your References section:

    Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Sheff MA, Thorn KS. Yeast 2004 Jun;21(8):661-70. 10.1002/yea.1130 PubMed 15197731
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