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pMSCVpuro-Mito-DsRed1
(Plasmid #87379)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87379 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCVpuro
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6264
  • Total vector size (bp) 7260
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TXN2 mitochondrial transit peptide
  • Alt name
    Thioredoxin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    180
  • Mutation
    First 60 aa only
  • GenBank ID
    NM_012473.3
  • Entrez Gene
    TXN2 (a.k.a. COXPD29, MT-TRX, MTRX, TRX2, TXN)
  • Promoter Retroviral 5' LTR promoter
  • Tag / Fusion Protein
    • DsRed1 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pMSCV-F
  • 3′ sequencing primer pMSCV-R
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    mito-DsRed1 was obtained from Prof. Du Feng (Guangzhou Medical University, China)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCVpuro-Mito-DsRed1 was a gift from Björn Stork (Addgene plasmid # 87379 ; http://n2t.net/addgene:87379 ; RRID:Addgene_87379)

  • For your References section:

    The mycotoxin phomoxanthone A disturbs the form and function of the inner mitochondrial membrane. Bohler P, Stuhldreier F, Anand R, Kondadi AK, Schlutermann D, Berleth N, Deitersen J, Wallot-Hieke N, Wu W, Frank M, Niemann H, Wesbuer E, Barbian A, Luyten T, Parys JB, Weidtkamp-Peters S, Borchardt A, Reichert AS, Pena-Blanco A, Garcia-Saez AJ, Itskanov S, van der Bliek AM, Proksch P, Wesselborg S, Stork B. Cell Death Dis. 2018 Feb 19;9(3):286. doi: 10.1038/s41419-018-0312-8. 10.1038/s41419-018-0312-8 PubMed 29459714
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