pCG004
(Plasmid #87377)

• Purpose: ORF part assembly entry vector derived from pHT01.
• Depositing Lab: Tom Ellis
• Publication: Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292.

Backbone

• Vector backbone: pHT01
• Backbone manufacturer: MoBioTec
• Total vector size (bp): 9026
• Modifications to backbone: Addition of GFP dropout part for BsaI golden gate and removal of backbone BsaI sites.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: N/A
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP dropout part

Resource Information

• Supplemental Documents:
  • pcg004.gb
• A portion of this plasmid was derived from a plasmid made by: The plasmid from which pCG004 was generated was obtained from MoBioTec, which required an MTA.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCG004 is identical to pHT01 except for two changes. Firstly an unwanted backbone BsaI site with the AmpR cassette was removed. Secondly, the multiple-cloning site of pHT01 was replaced with a BsaI dropout part. This part consists of a constitutive GFP mut3b-expression cassette (using the Pveg promoter, spoVG RBS and rrnB terminator) flanked by BsaI restriction sites. During assembly of ORF parts into pCG004, the GFP-expression cassette is removed and the full-length ORF inserted downstream of the RBS and upstream of the terminator.