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Addgene

pCG004
(Plasmid #87377)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87377 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pHT01
  • Backbone manufacturer
    MoBioTec
  • Total vector size (bp) 9026
  • Modifications to backbone
    Addition of GFP dropout part for BsaI golden gate and removal of backbone BsaI sites.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    N/A
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP dropout part

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The plasmid from which pCG004 was generated was obtained from MoBioTec, which required an MTA.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pCG004 is identical to pHT01 except for two changes. Firstly an unwanted backbone BsaI site with the AmpR cassette was removed. Secondly, the multiple-cloning site of pHT01 was replaced with a BsaI dropout part. This part consists of a constitutive GFP mut3b-expression cassette (using the Pveg promoter, spoVG RBS and rrnB terminator) flanked by BsaI restriction sites. During assembly of ORF parts into pCG004, the GFP-expression cassette is removed and the full-length ORF inserted downstream of the RBS and upstream of the terminator.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCG004 was a gift from Tom Ellis (Addgene plasmid # 87377 ; http://n2t.net/addgene:87377 ; RRID:Addgene_87377)
  • For your References section:

    Extracellular Self-Assembly of Functional and Tunable Protein Conjugates from Bacillus subtilis. Gilbert C, Howarth M, Harwood CR, Ellis T. ACS Synth Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292. 10.1021/acssynbio.6b00292 PubMed 28230977