pCG-D-mSpyCatcher-His6-E
(Plasmid #87376)

• Purpose: An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF ass
• Depositing Lab: Tom Ellis
• Publication: Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292.

Backbone

• Vector backbone: pYTK001
• Backbone size w/o insert (bp): 2676
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mSpyCatcher
• Alt name: SpyCatcher deltaN1 deltaC2
• Tag / Fusion Protein:
  • His6 tag (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pcg-d-mspycatcher-e.gb
• A portion of this plasmid was derived from a plasmid made by: The DNA sequence encoding the SpyCatcher domain was obtained from Oxford University, which required an MTA.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry