pCG-B-mSpyCatcher-C
(Plasmid
#87374)
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PurposeAn ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87374 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYTK001
- Backbone size w/o insert (bp) 2676
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemSpyCatcher
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Alt nameSpyCatcher deltaN1 deltaC2
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThe DNA sequence encoding the SpyCatcher domain was obtained from Oxford University, which required an MTA.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCG-B-mSpyCatcher-C was a gift from Tom Ellis (Addgene plasmid # 87374 ; http://n2t.net/addgene:87374 ; RRID:Addgene_87374) -
For your References section:
Extracellular Self-Assembly of Functional and Tunable Protein Conjugates from Bacillus subtilis. Gilbert C, Howarth M, Harwood CR, Ellis T. ACS Synth Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292. 10.1021/acssynbio.6b00292 PubMed 28230977
