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pEGFP-C1-Nup35
(Plasmid #87342)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87342 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nucleoporin 35
  • Alt name
    nucleoporin 35
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    981
  • GenBank ID
    BC047029
  • Entrez Gene
    NUP35 (a.k.a. MP-44, MP44, NP44, NUP53)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1-Nup35 was a gift from Lei Lu (Addgene plasmid # 87342 ; http://n2t.net/addgene:87342 ; RRID:Addgene_87342)

  • For your References section:

    The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex. Tie HC, Madugula V, Lu L. Biochem Biophys Res Commun. 2016 Sep 30;478(4):1694-9. doi: 10.1016/j.bbrc.2016.09.005. Epub 2016 Sep 6. 10.1016/j.bbrc.2016.09.005 PubMed 27613095
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