pEGFP-C1-Nup35-mut
(Plasmid #87341)

• Purpose: To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA
• Depositing Lab: Lei Lu
• Publication: Tie et al Biochem Biophys Res Commun. 2016 Sep 30;478(4):1694-9. doi: 10.1016/j.bbrc.2016.09.005. Epub 2016 Sep 6.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4731
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NUP35
• Alt name: Nucleoporin 35
• Species: H. sapiens (human)
• Insert Size (bp): 981
• Mutation: shRNA resistant changes
• GenBank ID: BC047029
• Entrez Gene: NUP35 (a.k.a. MP-44, MP44, NP44, NUP53)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry