pEGFP-C1-KSENB-Nup133-mut
(Plasmid #87339)

• Purpose: To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA
• Depositing Lab: Lei Lu
• Publication: Tie et al Biochem Biophys Res Commun. 2016 Sep 30;478(4):1694-9. doi: 10.1016/j.bbrc.2016.09.005. Epub 2016 Sep 6.

Backbone

• Vector backbone: pEGFP-C1-KSENB
• Backbone manufacturer: Clontech
• Modifications to backbone: multiple cloning sites of pEGFP-C1 have been modified
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NUP133
• Alt name: Nucleoporin 133
• Species: H. sapiens (human)
• Insert Size (bp): 3471
• Mutation: shRNA resistant changes; deletion of Nup133 AA1-7
• GenBank ID: BC020107.1
• Entrez Gene: NUP133 (a.k.a. GAMOS8, NPHS18, hNUP133)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ScaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry