pSUPER-retro-puro-shNup133-1
(Plasmid
#87334)
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PurposeTo express shRNA against human Nup133
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87334 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSUPER.retro.puro
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Backbone manufacturerOligoEngine
- Backbone size w/o insert (bp) 6349
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Vector typeMammalian Expression, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameshRNA against human Nup133
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gRNA/shRNA sequencetgttcagggacaaatataa
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SpeciesH. sapiens (human)
- Promoter H1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bglll (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer GGAAGCCTTGGCTTTTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSUPER-retro-puro-shNup133-1 was a gift from Lei Lu (Addgene plasmid # 87334 ; http://n2t.net/addgene:87334 ; RRID:Addgene_87334) -
For your References section:
The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex. Tie HC, Madugula V, Lu L. Biochem Biophys Res Commun. 2016 Sep 30;478(4):1694-9. doi: 10.1016/j.bbrc.2016.09.005. Epub 2016 Sep 6. 10.1016/j.bbrc.2016.09.005 PubMed 27613095
