pSUPER-retro-puro-shNup88-HindIII
(Plasmid
#87329)
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PurposeTo express shRNA against human Nup88
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87329 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemodified pSUPER.neo+GFP
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Backbone manufacturerOligoEngine
- Backbone size w/o insert (bp) 6349
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Modifications to backboneWe removed the sequence between NheI and XmaI of pSUPER.neo+GFP and ligate a puromycin resistance gene flanked by NheI and XmaI into the vector. The product of this modified pSUPER.neo+GFP is used for our shRNAs cloning. All the annealed shRNA oligos were cloned between BglII and XhoI sites. BglII site is abolished after successful ligation.
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Vector typeMammalian Expression, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameshRNA against human Nup88
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gRNA/shRNA sequencetctgaaaaggacatagccc
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SpeciesH. sapiens (human)
- Promoter H1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bglll (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer GGAAGCCTTGGCTTTTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSUPER-retro-puro-shNup88-HindIII was a gift from Lei Lu (Addgene plasmid # 87329 ; http://n2t.net/addgene:87329 ; RRID:Addgene_87329) -
For your References section:
The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex. Tie HC, Madugula V, Lu L. Biochem Biophys Res Commun. 2016 Sep 30;478(4):1694-9. doi: 10.1016/j.bbrc.2016.09.005. Epub 2016 Sep 6. 10.1016/j.bbrc.2016.09.005 PubMed 27613095