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pSUPER-retro-puro-shNup88-HindIII
(Plasmid #87329)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87329 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified pSUPER.neo+GFP
  • Backbone manufacturer
    OligoEngine
  • Backbone size w/o insert (bp) 6349
  • Modifications to backbone
    We removed the sequence between NheI and XmaI of pSUPER.neo+GFP and ligate a puromycin resistance gene flanked by NheI and XmaI into the vector. The product of this modified pSUPER.neo+GFP is used for our shRNAs cloning. All the annealed shRNA oligos were cloned between BglII and XhoI sites. BglII site is abolished after successful ligation.
  • Vector type
    Mammalian Expression, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    shRNA against human Nup88
  • gRNA/shRNA sequence
    tctgaaaaggacatagccc
  • Species
    H. sapiens (human)
  • Promoter H1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bglll (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GGAAGCCTTGGCTTTTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUPER-retro-puro-shNup88-HindIII was a gift from Lei Lu (Addgene plasmid # 87329 ; http://n2t.net/addgene:87329 ; RRID:Addgene_87329)

  • For your References section:

    The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex. Tie HC, Madugula V, Lu L. Biochem Biophys Res Commun. 2016 Sep 30;478(4):1694-9. doi: 10.1016/j.bbrc.2016.09.005. Epub 2016 Sep 6. 10.1016/j.bbrc.2016.09.005 PubMed 27613095
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