pHA-N1-GalT
(Plasmid #87326)

• Purpose: To express GalT-HA in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
• Depositing Lab: Lei Lu
• Publication: Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15-09-0664.

Backbone

• Vector backbone: pHA-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4030
• Total vector size (bp): 4273
• Modifications to backbone: mCherry is replaced with HA, which is generated by annealed oligos flanking with BamHI and NotI.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 1-81 amino acids from β1,4-galactosyltransferase
• Species: H. sapiens (human)
• Insert Size (bp): 243
• GenBank ID: NM_001497.3
• Entrez Gene: B4GALT1 (a.k.a. B4GAL-T1, CDG2D, CLDLFIB, GGTB2, GT1, GTB, beta4Gal-T1)
• Promoter: CMV-F
• Tag / Fusion Protein:
  • HA (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry