pAcGFP1-N1-SialT
(Plasmid #87324)

• Purpose: To express SialT-AcGFP1 in mammalian cells, SialT encodes the full length coding sequence of β-galactosamide α-2,6-sialyltransferase
• Depositing Lab: Lei Lu
• Publication: Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15-09-0664.

Backbone

• Vector backbone: pAcGFP1-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5921
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ST 6 beta-galactoside alpha-2,6-sialyltransferase 1
• Species: H. sapiens (human)
• Insert Size (bp): 1221
• GenBank ID: NM_003032
• Entrez Gene: ST6GAL1 (a.k.a. CDw75, SIAT1, ST6GalI, ST6N)
• Promoter: CMV
• Tag / Fusion Protein:
  • AcGFP1 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry