pSGDluc
(Plasmid
#87323)
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Purpose(Empty Backbone) This vector has been discontinued as of 1/16/2019. See Comments below for information on the updated version of the pSGDluc vector.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87323 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBR322
- Backbone size (bp) 5853
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Vector typeMammalian Expression, Luciferase
- Promoter SV40 and T7
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
As of 1/16/2019, this vector has been discontinued, but should function as described in the associated publication.
An updated version of the pSGDluc vector can be found here: https://www.addgene.org/119760/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSGDluc was a gift from John Atkins (Addgene plasmid # 87323) -
For your References section:
Avoidance of reporter assay distortions from fused dual reporters. Loughran G, Howard MT, Firth AE, Atkins JF. RNA. 2017 Apr 25. pii: rna.061051.117. doi: 10.1261/rna.061051.117. 10.1261/rna.061051.117 PubMed 28442579
