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Addgene

pSGDluc
(Plasmid #87323)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87323 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBR322
  • Backbone size (bp) 5853
  • Vector type
    Mammalian Expression, Luciferase
  • Promoter SV40 and T7

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

As of 1/16/2019, this vector has been discontinued, but should function as described in the associated publication.

An updated version of the pSGDluc vector can be found here: https://www.addgene.org/119760/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSGDluc was a gift from John Atkins (Addgene plasmid # 87323)

  • For your References section:

    Avoidance of reporter assay distortions from fused dual reporters. Loughran G, Howard MT, Firth AE, Atkins JF. RNA. 2017 Apr 25. pii: rna.061051.117. doi: 10.1261/rna.061051.117. 10.1261/rna.061051.117 PubMed 28442579
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