PumA
(Plasmid #87319)

• Purpose: PumHD w/each unit mutated to bind A (as in Fig. 1C; unit 6 is the Pumby for A)
• Depositing Lab: Edward Boyden
• Publication: Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2579-88. doi: 10.1073/pnas.1519368113. Epub 2016 Apr 26.

Backbone

• Vector backbone: pCI
• Backbone manufacturer: Promega
• Modifications to backbone: mutated to remove BsaI, original promoter replaced with UBC promoter
• Vector type: Mammalian Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pumilio protein mutant
• Alt name: PumA
• Species: H. sapiens (human)
• Insert Size (bp): 1062
• Mutation: all 8 units mutated to bind A
• Entrez Gene: PUM1 (a.k.a. HSPUM, NEDMSF, PUMH, PUMH1, PUML1, SCA47)
• Promoter: UBC

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TAATACGACTCACTATAGG
• 3′ sequencing primer: GCGTCCGCCTAAGTCA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry