pRS414 Hsh155 N295D/H331D
(Plasmid
#87285)
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PurposeEncodes Hsh155 N295D H331D for expression in yeast
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87285 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS414
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Backbone manufacturerATCC
- Backbone size w/o insert (bp) 4788
- Total vector size (bp) 8124
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHSH155
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)3426
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MutationN295D & H331D
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GenBank IDNP_014015.1
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Entrez GeneHSH155 (a.k.a. YMR288W)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer TGTAAAACGACGGCCAGT
- 3′ sequencing primer CAGGAAACAGCTATGAC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS414 Hsh155 N295D/H331D was a gift from Aaron Hoskins (Addgene plasmid # 87285 ; http://n2t.net/addgene:87285 ; RRID:Addgene_87285) -
For your References section:
SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast. Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA. Nucleic Acids Res. 2017 Jan 6. pii: gkw1349. doi: 10.1093/nar/gkw1349. 10.1093/nar/gkw1349 PubMed 28062854