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HA-HIF1α P402A/P564A/N803A-pcDNA3
(Plasmid #87261)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87261 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.0
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5433
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hypoxia inducible factor 1 alpha
  • Alt name
    HIF1a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2505
  • Mutation
    P402A/P564A/N803A
  • GenBank ID
    NM_001530
  • Entrez Gene
    HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pcDNA3.0-HA-HIF1α P402A/P564A/N803A was made from pcDNA3.0-HA-HIF1α P402A/P564A ( Addgene (Plasmid #18955) using the QuikChange site-directed mutagenesis kit (Stratagene)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-HIF1α P402A/P564A/N803A-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 87261)

  • For your References section:

    The hypoxia-inducible factor 2alpha N-terminal and C-terminal transactivation domains cooperate to promote renal tumorigenesis in vivo. Yan Q, Bartz S, Mao M, Li L, Kaelin WG. Mol Cell Biol. 2007 Mar . 27(6):2092-102. 10.1128/MCB.01514-06 PubMed 17220275
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