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Addgene

pEGFP-MYO10-HMM
(Plasmid #87256)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87256 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7540
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MYO10
  • Alt name
    Unconventional myosin 10
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2823
  • Mutation
    Myo10 is truncated to contain aa.1-941
  • GenBank ID
    NM_019472.2
  • Entrez Gene
    Myo10 (a.k.a. AW048724, D15Ertd600, D15Ertd600e, mKIAA0799, myos, myosin-X)
  • Promoter CMV

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV_fwd_primer
  • 3′ sequencing primer EBV_rev_primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-MYO10-HMM was a gift from Thomas Friedman (Addgene plasmid # 87256 ; http://n2t.net/addgene:87256 ; RRID:Addgene_87256)

  • For your References section:

    Harnessing Molecular Motors for Nanoscale Pulldown in Live Cells. Bird JE, Barzik M, Drummond MC, Sutton DC, Goodman SM, Morozko EL, Cole SM, Boukhvalova AK, Skidmore J, Syam D, Wilson EA, Fitzgerald T, Rehman AU, Martin DM, Boger ET, Belyantseva IA, Friedman TB. Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-08-0583. 10.1091/mbc.E16-08-0583 PubMed 27932498
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