pcDNA3.1-MYO10-HMM-Nanotrap
(Plasmid #87255)

• Purpose: Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.
• Depositing Lab: Thomas Friedman
• Publication: Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-08-0583.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Thermo Fisher
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 8589
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MYO10
• Alt name: Unconventional myosin 10
• Species: M. musculus (mouse)
• Insert Size (bp): 2823
• Mutation: Myo10 is truncated to contain aa.1-941
• GenBank ID: NM_019472.2
• Entrez Gene: Myo10 (a.k.a. AW048724, D15Ertd600, D15Ertd600e, mKIAA0799, myos, myosin-X)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP-nanotrap (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV_fwd_primer
• 3′ sequencing primer: BGH_rev_primer

Resource Information

• Supplemental Documents:
  • pcDNA3.1-Myo10-HMM-Nanotrap.gbk
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry