pLenti-CaMKIIa-mTagBFP2
(Plasmid #87254)

• Purpose: Lentiviral fluorescent reporter using the neuronal cell type-specific promoter of the CaM Kinase II alpha gene.
• Depositing Lab: James Ellis
• Publication: pLenti-CaMKIIalpha-mTagBFP2 (unpublished)

Backbone

• Vector backbone: pLenti-CaMKIIa-GFP
• Backbone size w/o insert (bp): 9926
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mTagBFP2
• Species: Synthetic
• Insert Size (bp): 738
• Promoter: CaMKIIa
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CaMKIIa promoter-based primer
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Insert was synthesized as gBlock at IDT. Sequence was copied from Addgene plasmid 34632. Vector backbone was provided by Oleksandr Shcheglovitov (University of Utah).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry