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Addgene

pCM184-ParB2::eGFP
(Plasmid #87252)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87252 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCM184
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ParB2
  • Species
    Burkholderia cenocepacia
  • Promoter pBAD/tetO
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCM184-ParB2::eGFP was a gift from Kerstin Bystricky (Addgene plasmid # 87252 ; http://n2t.net/addgene:87252 ; RRID:Addgene_87252)
  • For your References section:

    DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells. Saad H, Gallardo F, Dalvai M, Tanguy-le-Gac N, Lane D, Bystricky K. PLoS Genet. 2014 Mar 13;10(3):e1004187. doi: 10.1371/journal.pgen.1004187. eCollection 2014 Mar. PGENETICS-D-13-01770 [pii] PubMed 24625580