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Purposegene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-Hph
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87251 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAG32
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHyg
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Alt nameresistance to hygromycin
- Promoter TEF
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Tag
/ Fusion Protein
- parS segments (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFG4 was a gift from Kerstin Bystricky (Addgene plasmid # 87251 ; http://n2t.net/addgene:87251 ; RRID:Addgene_87251) -
For your References section:
DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells. Saad H, Gallardo F, Dalvai M, Tanguy-le-Gac N, Lane D, Bystricky K. PLoS Genet. 2014 Mar 13;10(3):e1004187. doi: 10.1371/journal.pgen.1004187. eCollection 2014 Mar. PGENETICS-D-13-01770 [pii] PubMed 24625580
