pANCH2-Nat
(Plasmid
#87249)
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Purposegene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAG25
- Total vector size (bp) 4840
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNat
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Alt nameresistance to nourseothricin
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pANCH2-Nat was a gift from Kerstin Bystricky (Addgene plasmid # 87249 ; http://n2t.net/addgene:87249 ; RRID:Addgene_87249) -
For your References section:
DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells. Saad H, Gallardo F, Dalvai M, Tanguy-le-Gac N, Lane D, Bystricky K. PLoS Genet. 2014 Mar 13;10(3):e1004187. doi: 10.1371/journal.pgen.1004187. eCollection 2014 Mar. PGENETICS-D-13-01770 [pii] PubMed 24625580