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Addgene

pFlagCMV-mCARcyt-SIV
(Plasmid #87237)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87237 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFlag-CMV
  • Backbone manufacturer
    Sigma
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CXADR
  • Alt name
    coxsackievirus_adenovirus receptor
  • Alt name
    CAR
  • Species
    M. musculus (mouse)
  • Mutation
    SIV isoform
  • Entrez Gene
    Cxadr (a.k.a. 2610206D03Rik, CAR, MCAR, MCVADR)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFlagCMV-mCARcyt-SIV was a gift from Fritz Rathjen (Addgene plasmid # 87237 ; http://n2t.net/addgene:87237 ; RRID:Addgene_87237)
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