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Addgene

pGEX-chCARex
(Plasmid #87233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-1
  • Backbone manufacturer
    ATCC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CXADR
  • Alt name
    coxsackievirus_adenovirus receptor
  • Alt name
    CAR
  • Species
    G. gallus (chicken)
  • Entrez Gene
    CXADR (a.k.a. TSTD1)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer pGEX5
  • 3′ sequencing primer pGEX3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-chCARex was a gift from Fritz Rathjen (Addgene plasmid # 87233 ; http://n2t.net/addgene:87233 ; RRID:Addgene_87233)

  • For your References section:

    The coxsackievirus-adenovirus receptor reveals complex homophilic and heterophilic interactions on neural cells. Patzke C, Max KE, Behlke J, Schreiber J, Schmidt H, Dorner AA, Kroger S, Henning M, Otto A, Heinemann U, Rathjen FG. J Neurosci. 2010 Feb 24;30(8):2897-910. doi: 10.1523/JNEUROSCI.5725-09.2010. 10.1523/JNEUROSCI.5725-09.2010 PubMed 20181587
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