pSG5-chCAR
(Plasmid
#87231)
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PurposeMammalian expression of chicken CAR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87231 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSG5
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Backbone manufacturerStratagene
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCXADR
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Alt namecoxsackievirus_adenovirus receptor
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Alt nameCAR
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SpeciesG. gallus (chicken)
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Entrez GeneCXADR (a.k.a. TSTD1)
- Promoter SV40
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG5-chCAR was a gift from Fritz Rathjen (Addgene plasmid # 87231 ; http://n2t.net/addgene:87231 ; RRID:Addgene_87231) -
For your References section:
The coxsackievirus-adenovirus receptor reveals complex homophilic and heterophilic interactions on neural cells. Patzke C, Max KE, Behlke J, Schreiber J, Schmidt H, Dorner AA, Kroger S, Henning M, Otto A, Heinemann U, Rathjen FG. J Neurosci. 2010 Feb 24;30(8):2897-910. doi: 10.1523/JNEUROSCI.5725-09.2010. 10.1523/JNEUROSCI.5725-09.2010 PubMed 20181587