hisSUMO-Gli1
(Plasmid #87225)

• Purpose: Express hisSUMO tagged DNA binding domain of Gli1(mouse) in E. coli
• Depositing Lab: Gary Stormo
• Publication: Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi: 10.1126/sciadv.aao1799. eCollection 2017 Nov.

Backbone

• Vector backbone: NEB DHFR control plasmid
• Total vector size (bp): 3117
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GLI1
• Species: M. musculus (mouse)
• Insert Size (bp): 519
• Entrez Gene: Gli1 (a.k.a. Zfp-5, Zfp5)
• Promoter: T7
• Tag / Fusion Protein:
  • hisSUMO (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • hisSUMO-Gli1(mouse).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The DNA binding domain of zinc finger protein GLI1 in mouse genome, used for expression in E. coli.

Note: There is a K58R mutation in SUMO in this plasmid, the depositor has confirmed that it does not affect plasmid function.